s6 ribosomal protein polyclonal rabbit  (Cell Signaling Technology Inc)

Journal: The Journal of Physiology

Article Title: Muscle regeneration is improved by hot water immersion but unchanged by cold following a simulated musculoskeletal injury in humans

doi: 10.1113/jp287777

Figure Lengend Snippet: Figure 9. Representative blots and quantification of phosphorylated mTOR (A, B), phosphorylated-S6 (C, D) and phosphorylated-p70 (E, F) protein expression at PRE, D5 and D11 in CWI, TWI and HWI groups Data are presented as medians along with individual data. #Significantly different from PRE (P < 0.05); ##significantly different from PRE (P < 0.01); †significantly different from D5 (P < 0.05); ∗significantly different between groups (P < 0.05).

Article Snippet: The membranes were incubated overnight at 4°C with the following primary antibodies: rabbit polyclonal phosphorylated nuclear factor-κB (NF-κB) p65 (1:1000, cat. no. 3033, Cell Signaling Technology, Danvers, MA, USA), monoclonal rat interleukin (IL)-10 (1:500, sc-73309, Santa Cruz Biotechnology, Dallas, TX, USA), mouse monoclonal HSP70 (1:750, ADI-SPA-810, Enzo, Farmingdale, NY, USA), mouse monoclonal HSP27 (1:750, ADI-SPA-800, Enzo Life Sciences, Farmingdale, NY, USA), rabbit polyclonal phosphorylated mammalian target of rapamycin (mTOR; 1:1000, cat. no. 2971, Cell Signaling Technology), rabbit polyclonal phosphorylated S6 ribosomal protein (1:1000, cat. no. 5364, Cell Signaling Technology), mousemonoclonal p-p70 S6 kinase (1:1000, cat. no. 9206, Cell Signaling Technology) rabbit polyclonal vascular endothelial growth factor (VEGF; 1:500, sc507, Santa Cruz Biotechnology) and rabbit polyclonal transforming growth factor β (TGF-β 1:1000, cat. no. 3711, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing

Journal: bioRxiv

Article Title: GCS-H2 is essential for growth as it acts as the main relay for mitochondrial lipoylation in heterotrophic tissues of Arabidopsis thaliana

doi: 10.1101/2024.10.14.617781

Figure Lengend Snippet: (a) Primary root growth in 10 day-old WT-Col0 and h2-1 plants grown under long-day conditions; scale bars = 1cm; and (b) quantification of root growth over time for WT (Col-0) and h2-1 plants grown in light. (c ) Primary root and hypocotyl growth in seedlings etiolated for 10 days; scale bars = 1cm; and ( d ) quantification of root and hypocotyl growth over time for WT (Col-0) and h2-1 plants grown in darkness. ( e ) Cell division in WT and h2-1 root tips reported by fluorescence of GFP in root tips of 8-day-old plantlets transformed with pCycB1- eGFP ; scale bar = 40 µm. Region of Interest (ROI) was defined as being the root area from the quiescent center to 250 µm upward as shown by the orange area defined on the bright field pictures ( f ) Quantification of fluorescence in WT and h2-1 lines transformed with a pCycB1-eGFP (n=3; see SFig. 4), **: P value < 0.01 after Student’s t-test. ( g ) Immunoblot reporting the phosphorylation state of RPS6, target of TOR (whole gels and replicates can be found in SFig. 5). ( h ) quantification of TOR kinase activity with RPS6-P/RPS6 ratio, (n=5), ***: P < 0.001 value after Student’s t-test.

Article Snippet: Membranes were blocked with 5% milk in PBS-T for 1 h followed by an overnight incubation at 4°C with specific polyclonal primary antibodies anti- RPS6 (1/1000, Cell Signaling Technology, 2217S), anti-RPS6P (1/2000; ), diluted in 2 % milk in PBS-T. After 2 h incubation at room temperature with a goat anti-rabbit (for RPS6P) or a goat anti-mouse (for RPS6) secondary antibodies conjugated to horseradish peroxidase (1/2000 in 2 % milk in PBS-T, Agrisera, AS09 602 and AS11 1772).

Techniques: Fluorescence, Transformation Assay, Western Blot, Phospho-proteomics, Activity Assay